Journal: iScience

Article Title: APC-driven actin nucleation powers collective cell dynamics in colorectal cancer cells

doi: 10.1016/j.isci.2023.106583

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Article Snippet: Plasmid: GFP-alone , Addgene , Cat# 54759; RRID:Addgene_54759.

Techniques: Recombinant, Modification, Saline, Stable Transfection, Expressing, Plasmid Preparation, Software, Microscopy, Chemotaxis Assay, Migration

Journal: EMBO reports

Article Title: Extended-Synaptotagmin-1 and -2 control T cell signaling and function.

doi: 10.1038/s44319-023-00011-7

Figure Lengend Snippet: Figure 4. E-Syt proteins modulate DAG and signaling markers in resting-state Jurkat cells.

Article Snippet: For rescue experiments, we electroporated EGFP-ESyt1, EGFP-E-Syt2, and EGFP-LifeAct plasmids into the respective E-Syt KO Jurkat cells and WT-Jurkat cells for control. pCMV-EGFP-PKCg-C1 was used for DAG detection (Addgene plasmid #112266). pmCherry-C1ab-Prkd1 was a gift from Gerry Hammond and Tamas Balla (Addgene plasmid # 139314; http:// n2t.net/addgene:139314; RRID:Addgene_139314). mCherry-Lifeact and EGFP-Lifeact were also used for actin controls. mCherry-ESyt1 and mCherry-E-Syt2 were generated in the lab. mCherry-ESyt1 was generated by using the SacI and SalI restriction sites on EGFP-E-Syt1 followed by ligation to the mCherry-C1 plasmid. mCherry-E-Syt2 was generated by PCR amplification of pCR4TOPO-E-Syt2 using BspEI an SalI primers.

Techniques: